Biotechnological aspects of selection of yeast biodegradants for the purpose of biological purification of soil from motor oil

UDK: 606:502.521:665.7
DOI: 10.24887/0028-2448-2020-12-147-150
Key words: motor oil, yeast biodegradants, biodegradation of oil and oil products, mycological enzymes, biotoxicity
Authors: M.G. Chesnokova (Omsk State Technical University, RF, Omsk; Omsk State Medical University, RF, Omsk), V.M. Chugunov (Branch of Gazpromneft-Lubricants LLC Omsk Plant of Lubricants, RF, Omsk)

The article is devoted to the assessment of biotechnological aspects of the selection of mycological aboriginal biodestructors for biological cleaning of soil from engine oil, which is relevant in the development of biotechnologies for remediation of the environment from oil and oil products pollution, the search and isolation of micro- and mycobiota strains developing in regional soil and climatic conditions, with strain specificity of transformation of aromatic hydrocarbons.

Yeast-like fungi of the genus Candida were isolated on Sabouraud's medium; for their selective isolation, Candi Select 4 medium (BioRad, France) was used, which provides direct identification of yeast-like fungi species. The mycological identification of cultures was carried out on the basis of studying the characteristic morphological, cultural, and biochemical properties. The authors developed technological regimes for the cultivation of strains-destructors with the aim of obtaining cell biomass for asporogenic yeast cultures using a number of nutrient media. Samples of soil contaminated with engine oil and control samples were taken; biotoxicity, catalase and cellulase activity were studied. Selected after mycological isolation of the culture of fungi, a weak degree of enrichment with the enzyme catalase in soil samples contaminated with motor oil, strongly pronounced biotoxicity and cellulase activity were established. The paper proposes the use of selected yeast microorganisms, which are active biodestructors, resistant to high concentrations of motor oil oil, to create a microbial consortium when carrying out biological treatment of soil contaminated with motor oil.


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