Biotechnological aspects of selection of yeast biodegradants for the purpose of biological purification of soil from motor oil

UDK: 606:502.521:665.7
DOI: 10.24887/0028-2448-2020-12-147-150
Key words: motor oil, yeast biodegradants, biodegradation of oil and oil products, mycological enzymes, biotoxicity
Authors: M.G. Chesnokova (Omsk State Technical University, RF, Omsk; Omsk State Medical University, RF, Omsk), V.M. Chugunov (Branch of Gazpromneft-Lubricants LLC Omsk Plant of Lubricants, RF, Omsk)

The article is devoted to the assessment of biotechnological aspects of the selection of mycological aboriginal biodestructors for biological cleaning of soil from engine oil, which is relevant in the development of biotechnologies for remediation of the environment from oil and oil products pollution, the search and isolation of micro- and mycobiota strains developing in regional soil and climatic conditions, with strain specificity of transformation of aromatic hydrocarbons.

Yeast-like fungi of the genus Candida were isolated on Sabouraud's medium; for their selective isolation, Candi Select 4 medium (BioRad, France) was used, which provides direct identification of yeast-like fungi species. The mycological identification of cultures was carried out on the basis of studying the characteristic morphological, cultural, and biochemical properties. The authors developed technological regimes for the cultivation of strains-destructors with the aim of obtaining cell biomass for asporogenic yeast cultures using a number of nutrient media. Samples of soil contaminated with engine oil and control samples were taken; biotoxicity, catalase and cellulase activity were studied. Selected after mycological isolation of the culture of fungi, a weak degree of enrichment with the enzyme catalase in soil samples contaminated with motor oil, strongly pronounced biotoxicity and cellulase activity were established. The paper proposes the use of selected yeast microorganisms, which are active biodestructors, resistant to high concentrations of motor oil oil, to create a microbial consortium when carrying out biological treatment of soil contaminated with motor oil.

References

1. Lashkhi V.L., A view upon the applied chemistry of motor oils (In Russ.), Mir nefteproduktov. Vestnik neftyanykh kompaniy, 2014, no. 2, pp. 23–27.

2. Vasil'eva G.K., Strizhakova E.R., Bocharnikova E.A. et al., Oil and petroleum products as soil pollutants. The technology of combined physical and biological treatment of contaminated soils (In Russ.), Rossiyskiy khimicheskiy zhurnal = Russian Journal of General Chemistry, 2013, V. 57, no. 1, pp. 79–104.

3. Yang M., Yang Y.S., Du X. et al., Fate and transport of petroleum hydrocarbons in vadose zone: Compound-specific natural attenuation, Water, Air & Soil Pollution, 2013, V. 224, no. 3, art. 1439, 14 p.

4. Kotrovskiy M.N., "Gazpromneft' - smazochnye materialy" – the most powerful engine oil production complex in Russia put into operation (In Russ.), Gornaya promyshlennost', 2014, no. 4(116), pp. 74–76.

5. Dzhavadov N.G., Eminov R.A., Mursalov N.Z. et al., Adaptive optimization method of bioremediation polluted oil and oil products soil plots (In Russ.), Problemy sbora, podgotovki i transporta nefti i nefteproduktov, 2019, no. № 1 (117), pp. 144–153.

6. Mohajeri L., Aziz H.A., Isa M.H., Ex-situ bioremediation of crude oil in soil, a comparative kinetic analysis, Bulletin of Environmental Contamination & Toxicology, 2010, V. 85, no. 1, pp. 54–58.

7. Khaustov A.P., Redina M.M., Transformations of oil damages in geological environment under the effect of liquid substance (In Russ.), Neft'. Gaz. Novatsii, 2013, no. 10, pp. 22–30.

8. Zemo D.A., O'Reilly K.T., Mohier R.E. et al., Life cycle of petroleum biodegradation metabolite plumes, and implications for risk management at fuel release sites, Integrated Environmental Assessment & Management, 2017, V. 13, no. 4, pp. 714–727.

9. Nilanjana D., Chandran P., Microbial degradation of petroleum hydrocarbon contaminants: an overview, Biotechnology Research International, 2011, V. 1, pp. 1–12.

10. Krasil'nikov P.A., Seredin V.V., Leonovich M.F., Investigation of the distribution of hydrocarbons to cut the soil mass (In Russ.), Fundamental'nye issledovaniya, 2015, no. 2, pp. 3100–3104. 

11. Amos R.T., Bekins B.A., Cozzarelli I.M. et al., Evidence for iron-mediated anaerobic methane oxidation in a crude oil-contaminated aquifer, Geobiology, 2012, V. 10, no. 6, pp. 506–517.

12. Ivanova A.E., Kanat'eva A.Yu., Kurganov A.A., Aerobic biodegradation of liquid motor fuels under extreme acidic conditions (In Russ.), Mikrobiologiya = Microbiology (Mikrobiologiya), 2019, V. 88, no. 3, pp. 318–327.

13. Chesnokova M.G., Shalay V.V., Kriga A.S., Biocorrosive activity analysis of the oil pipeline soil in the khanty-mansiysk autonomous region of Ugra and the Krasnodar territory of the Russian Federation, AIP Conference Proceedings, 2017, p. 020019, https:// doi.org/ 10.1063/1.4998839.

14. Chesnokova M.G., Shalay V.V., An actuality of soil micromyceta community studies for soil biocorrosive activity evaluation on the oil pipeline routes, AIP Conference Proceedings, 2018, p. 020006, URL: https://doi.org/10.1063/1.5051845.

15. Rojo F., Degradation of alkanes by bacteria, Environmental Microbiology, 2009, V. 11, no. 10, pp. 2477–2490.

The article is devoted to the assessment of biotechnological aspects of the selection of mycological aboriginal biodestructors for biological cleaning of soil from engine oil, which is relevant in the development of biotechnologies for remediation of the environment from oil and oil products pollution, the search and isolation of micro- and mycobiota strains developing in regional soil and climatic conditions, with strain specificity of transformation of aromatic hydrocarbons.

Yeast-like fungi of the genus Candida were isolated on Sabouraud's medium; for their selective isolation, Candi Select 4 medium (BioRad, France) was used, which provides direct identification of yeast-like fungi species. The mycological identification of cultures was carried out on the basis of studying the characteristic morphological, cultural, and biochemical properties. The authors developed technological regimes for the cultivation of strains-destructors with the aim of obtaining cell biomass for asporogenic yeast cultures using a number of nutrient media. Samples of soil contaminated with engine oil and control samples were taken; biotoxicity, catalase and cellulase activity were studied. Selected after mycological isolation of the culture of fungi, a weak degree of enrichment with the enzyme catalase in soil samples contaminated with motor oil, strongly pronounced biotoxicity and cellulase activity were established. The paper proposes the use of selected yeast microorganisms, which are active biodestructors, resistant to high concentrations of motor oil oil, to create a microbial consortium when carrying out biological treatment of soil contaminated with motor oil.

References

1. Lashkhi V.L., A view upon the applied chemistry of motor oils (In Russ.), Mir nefteproduktov. Vestnik neftyanykh kompaniy, 2014, no. 2, pp. 23–27.

2. Vasil'eva G.K., Strizhakova E.R., Bocharnikova E.A. et al., Oil and petroleum products as soil pollutants. The technology of combined physical and biological treatment of contaminated soils (In Russ.), Rossiyskiy khimicheskiy zhurnal = Russian Journal of General Chemistry, 2013, V. 57, no. 1, pp. 79–104.

3. Yang M., Yang Y.S., Du X. et al., Fate and transport of petroleum hydrocarbons in vadose zone: Compound-specific natural attenuation, Water, Air & Soil Pollution, 2013, V. 224, no. 3, art. 1439, 14 p.

4. Kotrovskiy M.N., "Gazpromneft' - smazochnye materialy" – the most powerful engine oil production complex in Russia put into operation (In Russ.), Gornaya promyshlennost', 2014, no. 4(116), pp. 74–76.

5. Dzhavadov N.G., Eminov R.A., Mursalov N.Z. et al., Adaptive optimization method of bioremediation polluted oil and oil products soil plots (In Russ.), Problemy sbora, podgotovki i transporta nefti i nefteproduktov, 2019, no. № 1 (117), pp. 144–153.

6. Mohajeri L., Aziz H.A., Isa M.H., Ex-situ bioremediation of crude oil in soil, a comparative kinetic analysis, Bulletin of Environmental Contamination & Toxicology, 2010, V. 85, no. 1, pp. 54–58.

7. Khaustov A.P., Redina M.M., Transformations of oil damages in geological environment under the effect of liquid substance (In Russ.), Neft'. Gaz. Novatsii, 2013, no. 10, pp. 22–30.

8. Zemo D.A., O'Reilly K.T., Mohier R.E. et al., Life cycle of petroleum biodegradation metabolite plumes, and implications for risk management at fuel release sites, Integrated Environmental Assessment & Management, 2017, V. 13, no. 4, pp. 714–727.

9. Nilanjana D., Chandran P., Microbial degradation of petroleum hydrocarbon contaminants: an overview, Biotechnology Research International, 2011, V. 1, pp. 1–12.

10. Krasil'nikov P.A., Seredin V.V., Leonovich M.F., Investigation of the distribution of hydrocarbons to cut the soil mass (In Russ.), Fundamental'nye issledovaniya, 2015, no. 2, pp. 3100–3104. 

11. Amos R.T., Bekins B.A., Cozzarelli I.M. et al., Evidence for iron-mediated anaerobic methane oxidation in a crude oil-contaminated aquifer, Geobiology, 2012, V. 10, no. 6, pp. 506–517.

12. Ivanova A.E., Kanat'eva A.Yu., Kurganov A.A., Aerobic biodegradation of liquid motor fuels under extreme acidic conditions (In Russ.), Mikrobiologiya = Microbiology (Mikrobiologiya), 2019, V. 88, no. 3, pp. 318–327.

13. Chesnokova M.G., Shalay V.V., Kriga A.S., Biocorrosive activity analysis of the oil pipeline soil in the khanty-mansiysk autonomous region of Ugra and the Krasnodar territory of the Russian Federation, AIP Conference Proceedings, 2017, p. 020019, https:// doi.org/ 10.1063/1.4998839.

14. Chesnokova M.G., Shalay V.V., An actuality of soil micromyceta community studies for soil biocorrosive activity evaluation on the oil pipeline routes, AIP Conference Proceedings, 2018, p. 020006, URL: https://doi.org/10.1063/1.5051845.

15. Rojo F., Degradation of alkanes by bacteria, Environmental Microbiology, 2009, V. 11, no. 10, pp. 2477–2490.


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