Investigating bioremediation and detoxification of oil-contaminated cuttings and soil using bacterial preparation Rekoyl

UDK: 628.516+622.323]:663.18
DOI: 10.24887/0028-2448-2017-2-108-111
Key words: oil-contaminated soil, remediation, detoxification, bacterial preparation
Authors: E.S. Belik, L.V. Rudakova, I.S. Glushankova, A.A. Surkov, Ya.I. Vaysman (Perm National Research Polytechnic University, RF, Perm), V.G. Berlizova (ProfStroyAlyans LLC, RF, Perm)
Bioremediation is a commonly used soil treatment technology in case of crude and petroleum derivatives contamination. This technology is based on the principle of self-purification of oil-contaminated soil and ground in presence of hydrocarbon oxidizing microorganisms form. Using special bacterial drugs is a promising direction for the intensification of the cleaning of the environment from oil with the help of microorganisms. This increases the efficiency of removal of petroleum hydrocarbons by increasing the number of hydrocarbon oxidizing microorganisms. Currently, these bacterial preparations developed enough. Bacterial preparations which reduce not only the amount of oil in the soil, but also the content of heavy metals are of great interest. The authors present a biological product Rekoyl which consists of a consortium of oxidizing microorganisms immobilized on brown coal. It is show that the bacterial preparation Rekoyl is effective for biodegradation of petroleum hydrocarbons (reduction of oil content in the 63-93 %). In addition, this product helps detoxify contaminated cuttings and soils from heavy metals (reduction of mobile forms of heavy metals cadmium, copper, lead, zinc, chromium at 29-66%). Bioremediation technology using bacterial preparation Rekoyl is developed for cleaning oil-contaminated soils and drill cuttings based on laboratory and pilot-scale studies. Experimentally determined that the dose of the biopreparation - 0.1 g/kg.

References

1. Vaysman Ya.I., Glushankova I.S., Rudakova L.V. et al., Development of

technology remediation soil contaminated by oil using GUMIKOM (In

Russ.), Neftyanoe khozyaystvo = Oil Industry, 2013, no. 10, pp. 128131.

2. Marchenko M.Yu., Shuktueva M.I., Vinokurov V.A., Krasnopolskaya L.M.,

Bioremediation of oil contaminated soil (In Russ.), Bashkirskiy khimicheskiy

zhurnal = Bashkir chemical journal, 2011, V. 18, no. 4, pp. 191195.

3. Pystina N.B., Listov E.L., Balakirev I.V. et al., Development of biosorbent

based on hydrocarbon oxidizing microorganisms immobilized on hydrophobized

peat (In Russ.), Gazovaya promyshlennost = GAS Industry of

Russia, 2013, no. 2 (686), pp. 8285.

4. Ivshina I.B., Krivoruchko A.V., Kuyukina M.S. et al., Bioremediation of hydrocarbon

and heavy metal contaminated soil using Rhodococcus biosurfactants

and immobilized rhodococ-cal cells (In Russ.), Agrarnyy vestnik

Urala, 2012, no. 8(100), pp. 6568.

5. Nazarko M.D., Shcherbakov V.G., Aleksandrova A.V., Prospects for the

use of microorganisms for the biodegradation of oil polluted soils (In Russ.),

Izvestiya vuzov. Pishchevaya tekhnologiya, 2004, no. 4, . 8991.

6. Yagafarova G.G., Akchurina L.R., Fedorova Yu.A., Yagafarova I.R., Novel

sorbent for water purification from oil pollution (In Russ.), Ekologiya i

promyshlennost Rossii, 2011, no. 12, pp. 3435.

7. Kalinin V., Bacteria against oil contamination (In Russ.), Neftyanoe

khozyaystvo = Oil Industry, 2015, no. 3, pp. 96-97.

8. Das N., Chandran P., Microbial degradation of petroleum hydrocarbon contaminants:

An overview, Biotechnology Research International, 2011, 13 p.

9. Tereshchenko N.N., Lushnikov S.V., Sposob stimulirovaniya aktivnosti

uglevodorodokislyayushchikh mikroorganizmov v pochve, zagryaznennoy

neftyu i nefteproduktami (Prospects for the use of microorganisms for the

biodegradation of oil polluted soils), Proceedings of 1st International congress

Biotekhnologiya sostoyanie i perspektivy razvitiya (Biotechnology

the state and prospects of development), Moscow, 2002, p. 476.

10. Grechishcheva N.Yu., Vzaimodeystvie gumusovykh kislot s poliyadernymi

aromaticheskimi uglevodorodami: khimicheskie i toksikologicheskie aspekty

(The interaction of humic acids with polynuclear aromatic hydrocarbons:

chemical and toxicological aspects): thesis of candidate of

chemical science, Moscow, 2000, 153 .

11. Dagurov A.V., Vliyanie gumatov na toksichnost uglevodorodov nefti

(Influence of humates on the toxicity of petroleum hydrocarbons): thesis of

candidate of biological science, Irkutsk, 2004.

12. Illarionov S.A. Transfomatsia uglevodorodov hefti v pochvakh guminoi

zony (Petroleum hydrocarbons transformation in humid zones soil): thesis of

doctor of biological science, Syktyvkar, 2006, 422 p.

13. Saleem Kayd Mokhammed Abdulla, Ispolzovanie guminovykh preparatov

dlya detoksikatsii i biodegradatsii neftyanogo zagryazneniya (The use of

humic preparations for detoxification and biodegradation of oil pollution):

thesis of candidate of chemical science, Moscow, 2003, 106 .

6. Ivanov A.A., Yudina N.V., Maltseva E.V., Matis E.Ya., The study biostimulating

and detoxifying properties of humic acids of various origins in a oilpolluted

soil (In Russ.), Khimiya rastitelnogo syrya, 2007, no. 1, pp. 99103. 
Bioremediation is a commonly used soil treatment technology in case of crude and petroleum derivatives contamination. This technology is based on the principle of self-purification of oil-contaminated soil and ground in presence of hydrocarbon oxidizing microorganisms form. Using special bacterial drugs is a promising direction for the intensification of the cleaning of the environment from oil with the help of microorganisms. This increases the efficiency of removal of petroleum hydrocarbons by increasing the number of hydrocarbon oxidizing microorganisms. Currently, these bacterial preparations developed enough. Bacterial preparations which reduce not only the amount of oil in the soil, but also the content of heavy metals are of great interest. The authors present a biological product Rekoyl which consists of a consortium of oxidizing microorganisms immobilized on brown coal. It is show that the bacterial preparation Rekoyl is effective for biodegradation of petroleum hydrocarbons (reduction of oil content in the 63-93 %). In addition, this product helps detoxify contaminated cuttings and soils from heavy metals (reduction of mobile forms of heavy metals cadmium, copper, lead, zinc, chromium at 29-66%). Bioremediation technology using bacterial preparation Rekoyl is developed for cleaning oil-contaminated soils and drill cuttings based on laboratory and pilot-scale studies. Experimentally determined that the dose of the biopreparation - 0.1 g/kg.

References

1. Vaysman Ya.I., Glushankova I.S., Rudakova L.V. et al., Development of

technology remediation soil contaminated by oil using GUMIKOM (In

Russ.), Neftyanoe khozyaystvo = Oil Industry, 2013, no. 10, pp. 128131.

2. Marchenko M.Yu., Shuktueva M.I., Vinokurov V.A., Krasnopolskaya L.M.,

Bioremediation of oil contaminated soil (In Russ.), Bashkirskiy khimicheskiy

zhurnal = Bashkir chemical journal, 2011, V. 18, no. 4, pp. 191195.

3. Pystina N.B., Listov E.L., Balakirev I.V. et al., Development of biosorbent

based on hydrocarbon oxidizing microorganisms immobilized on hydrophobized

peat (In Russ.), Gazovaya promyshlennost = GAS Industry of

Russia, 2013, no. 2 (686), pp. 8285.

4. Ivshina I.B., Krivoruchko A.V., Kuyukina M.S. et al., Bioremediation of hydrocarbon

and heavy metal contaminated soil using Rhodococcus biosurfactants

and immobilized rhodococ-cal cells (In Russ.), Agrarnyy vestnik

Urala, 2012, no. 8(100), pp. 6568.

5. Nazarko M.D., Shcherbakov V.G., Aleksandrova A.V., Prospects for the

use of microorganisms for the biodegradation of oil polluted soils (In Russ.),

Izvestiya vuzov. Pishchevaya tekhnologiya, 2004, no. 4, . 8991.

6. Yagafarova G.G., Akchurina L.R., Fedorova Yu.A., Yagafarova I.R., Novel

sorbent for water purification from oil pollution (In Russ.), Ekologiya i

promyshlennost Rossii, 2011, no. 12, pp. 3435.

7. Kalinin V., Bacteria against oil contamination (In Russ.), Neftyanoe

khozyaystvo = Oil Industry, 2015, no. 3, pp. 96-97.

8. Das N., Chandran P., Microbial degradation of petroleum hydrocarbon contaminants:

An overview, Biotechnology Research International, 2011, 13 p.

9. Tereshchenko N.N., Lushnikov S.V., Sposob stimulirovaniya aktivnosti

uglevodorodokislyayushchikh mikroorganizmov v pochve, zagryaznennoy

neftyu i nefteproduktami (Prospects for the use of microorganisms for the

biodegradation of oil polluted soils), Proceedings of 1st International congress

Biotekhnologiya sostoyanie i perspektivy razvitiya (Biotechnology

the state and prospects of development), Moscow, 2002, p. 476.

10. Grechishcheva N.Yu., Vzaimodeystvie gumusovykh kislot s poliyadernymi

aromaticheskimi uglevodorodami: khimicheskie i toksikologicheskie aspekty

(The interaction of humic acids with polynuclear aromatic hydrocarbons:

chemical and toxicological aspects): thesis of candidate of

chemical science, Moscow, 2000, 153 .

11. Dagurov A.V., Vliyanie gumatov na toksichnost uglevodorodov nefti

(Influence of humates on the toxicity of petroleum hydrocarbons): thesis of

candidate of biological science, Irkutsk, 2004.

12. Illarionov S.A. Transfomatsia uglevodorodov hefti v pochvakh guminoi

zony (Petroleum hydrocarbons transformation in humid zones soil): thesis of

doctor of biological science, Syktyvkar, 2006, 422 p.

13. Saleem Kayd Mokhammed Abdulla, Ispolzovanie guminovykh preparatov

dlya detoksikatsii i biodegradatsii neftyanogo zagryazneniya (The use of

humic preparations for detoxification and biodegradation of oil pollution):

thesis of candidate of chemical science, Moscow, 2003, 106 .

6. Ivanov A.A., Yudina N.V., Maltseva E.V., Matis E.Ya., The study biostimulating

and detoxifying properties of humic acids of various origins in a oilpolluted

soil (In Russ.), Khimiya rastitelnogo syrya, 2007, no. 1, pp. 99103. 


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